关键词:biotech 简介:世界**品牌biotech原装**,质量保证,价格优惠。 我公司专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞治疗、临床应用等领域。国内范围内免费快递运输,如出现运输质量问题,我们可以包退换货。 产品介绍:biotech http://www.shijichina.com/goodsid/fenleisan/3444305/1.html biotech使用说明 1. 细胞的增殖和细胞毒实验,一般可在96孔细胞培养板中进行。 2. 每孔加入100微升细胞悬液。细胞的数量取决于实验目的和培养时间。增殖实验每孔通常加入103个以上数量的细胞;细胞毒性实验每孔至少加入5x103个或以上数量的细胞(具体每孔所用的细胞的数目,需根据细胞的大小,细胞增殖速度的快慢等因素确定)。 3. 接种的细胞按照实验需要,送入细胞培养箱进行培养。增殖实验通常要给予0-10微升特定的药物或生长因子进行刺激。细胞毒实验通常要给予0-10微升抑制因子或细胞毒药物; 4.培养结束后,取出阿尔玛蓝试剂,置室温融化混匀,于洁净工作台内按10微升/孔加入微孔板中,在细胞培养箱内继续孵育12-24小时,培养液颜色由蓝变为粉红(如采用荧光分光光度法,只需孵育5-8小时); 5.在570nm测定吸光度,参考波长600nm。如无此滤光片, 可用565 nm 和 610 nm的滤光片替代。 6.也可用荧光分光光度法检测,激发光波长在530-560 nm之间,发射光波长为590 nm。检测时间可在结果以荧光强度表示。 CP1100 Human PAI-1 Chromogenic Activity kit EP3010-1 Human PreAlbumin ELISA Kit EP1200-1 Huamn Plasminogen ELISA Kit EMP2211-1 Mouse Plasminogen ELISA Kit (Plasma/Serum Samples) EMP1200-1 Mouse Plasminogen ELISA Kit (Urine & Cell Culture Samples) EP1100-1 Human PAI-1 ELISA Kit EP1105-1 Human PAI-1/tPA ELISA Kit EP1311-1 Human Protein C ELSIA Kit (Plasma & Serum Samples) EP2312-1 Human Protein C ELSIA Kit (Urine & Cell Culture Samples) EP3022-1 Human Prothrombin ELSIA Kit EPP3022-1 Swine Prothrombin ELSIA Kit EMP3022-1 Mouse Prothrombin ELSIA Kit ER5010-1 Human Recoverin ELISA Kit ER1001-1 Human Resistin ELISA Kit EMR1001-1 Mouse Resistin ELISA Kit ER3005-1 Human RBP4 ELISA Kit ER1005-1 Human RBP ELISA Kit (Plasma & Serum Samples) ER2005-1 Human RBP ELISA Kit ES1001-1 Human SCF (Stem Cell Factor) ELISA Kit EMS1001-1 Mouse SCF (Stem Cell Factor) ELISA Kit ES2001-1 Human Survivin ELISA Kit ET3105-1 Human Transferrin ELISA Kit ( for Urine and Cell Culture Media) ET2105-1 Human Transferin ELISA Kit ERT2105-1 Rat Transferrin ELISA Kit ( for Plasma & Serum Samples) ERT3105-1 Rat Transferrin ELISA Kit ( for Urine and Cell Culture Media) ET1002-1 Human Tissue Factor ELISA Kit CT1002b Human TF Chromogenic Activity kit (Two step, Lipo) ET1005-1 Human TFPI ELISA Kit CT1001 Human tPA Chromogenic Activity Assay kit CT2002 Human tPA Chromogenic Activity Assay kit (Immuno-activity *****) ET1001-1 Human tPA ELISA Kit EMT1020-1 Mouse TAT Complexes ELISA Kit ET1020-1 Human TAT Complexes ELISA Kit ERT2010-1 Rat TNF-alpha ELISA Kit ET2010-1 Human Tumor Necrosis Factor Alpha (TNF-??) ELISA Kit EMT2010-1 Mouse TNF alpha ELISA Kit ET4010-1 Human Thrombin ELISA Kit ET3102-1 Human TGF-beta1 ELISA Kit CU1001b Human uPA Chromogenic Activity Assay kit (Indirect Assay) CU1001a Human uPA Chromogenic Activity Assay kit (Direct Assay)上一篇:biotech trading partners下一篇:Biotek Instruments Inc.
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