關鍵詞:biotech 簡介:世界**品牌biotech原裝**,質量保證,價格優惠。 我公司專業經營進口胎牛血清、細胞因子、ELISA試劑盒、細胞、抗體、生物試劑、耗材、培養基、一抗、二抗、其產品吸附均勻,吸附性好,空白值低,孔底透明度高,代做ELISA實驗等。產品涉及分子生物學、細胞生物學、細菌學、遺傳學、免疫學、生物化學、蛋白質學、細胞治療、臨床應用等領域。國內范圍內免費快遞運輸,如出現運輸質量問題,我們可以包退換貨。 產品介紹:biotech http://www.shijichina.com/goodsid/fenleisan/3444305/1.html biotech使用說明 1. 細胞的增殖和細胞毒實驗,一般可在96孔細胞培養板中進行。 2. 每孔加入100微升細胞懸液。細胞的數量取決于實驗目的和培養時間。增殖實驗每孔通常加入103個以上數量的細胞;細胞毒性實驗每孔至少加入5x103個或以上數量的細胞(具體每孔所用的細胞的數目,需根據細胞的大小,細胞增殖速度的快慢等因素確定)。 3. 接種的細胞按照實驗需要,送入細胞培養箱進行培養。增殖實驗通常要給予0-10微升特定的藥物或生長因子進行刺激。細胞毒實驗通常要給予0-10微升抑制因子或細胞毒藥物; 4.培養結束后,取出阿爾瑪藍試劑,置室溫融化混勻,于潔凈工作臺內按10微升/孔加入微孔板中,在細胞培養箱內繼續孵育12-24小時,培養液顏色由藍變為粉紅(如采用熒光分光光度法,只需孵育5-8小時); 5.在570nm測定吸光度,參考波長600nm。如無此濾光片, 可用565 nm 和 610 nm的濾光片替代。 6.也可用熒光分光光度法檢測,激發光波長在530-560 nm之間,發射光波長為590 nm。檢測時間可在結果以熒光強度表示。 CP1100 Human PAI-1 Chromogenic Activity kit EP3010-1 Human PreAlbumin ELISA Kit EP1200-1 Huamn Plasminogen ELISA Kit EMP2211-1 Mouse Plasminogen ELISA Kit (Plasma/Serum Samples) EMP1200-1 Mouse Plasminogen ELISA Kit (Urine & Cell Culture Samples) EP1100-1 Human PAI-1 ELISA Kit EP1105-1 Human PAI-1/tPA ELISA Kit EP1311-1 Human Protein C ELSIA Kit (Plasma & Serum Samples) EP2312-1 Human Protein C ELSIA Kit (Urine & Cell Culture Samples) EP3022-1 Human Prothrombin ELSIA Kit EPP3022-1 Swine Prothrombin ELSIA Kit EMP3022-1 Mouse Prothrombin ELSIA Kit ER5010-1 Human Recoverin ELISA Kit ER1001-1 Human Resistin ELISA Kit EMR1001-1 Mouse Resistin ELISA Kit ER3005-1 Human RBP4 ELISA Kit ER1005-1 Human RBP ELISA Kit (Plasma & Serum Samples) ER2005-1 Human RBP ELISA Kit ES1001-1 Human SCF (Stem Cell Factor) ELISA Kit EMS1001-1 Mouse SCF (Stem Cell Factor) ELISA Kit ES2001-1 Human Survivin ELISA Kit ET3105-1 Human Transferrin ELISA Kit ( for Urine and Cell Culture Media) ET2105-1 Human Transferin ELISA Kit ERT2105-1 Rat Transferrin ELISA Kit ( for Plasma & Serum Samples) ERT3105-1 Rat Transferrin ELISA Kit ( for Urine and Cell Culture Media) ET1002-1 Human Tissue Factor ELISA Kit CT1002b Human TF Chromogenic Activity kit (Two step, Lipo) ET1005-1 Human TFPI ELISA Kit CT1001 Human tPA Chromogenic Activity Assay kit CT2002 Human tPA Chromogenic Activity Assay kit (Immuno-activity *****) ET1001-1 Human tPA ELISA Kit EMT1020-1 Mouse TAT Complexes ELISA Kit ET1020-1 Human TAT Complexes ELISA Kit ERT2010-1 Rat TNF-alpha ELISA Kit ET2010-1 Human Tumor Necrosis Factor Alpha (TNF-??) ELISA Kit EMT2010-1 Mouse TNF alpha ELISA Kit ET4010-1 Human Thrombin ELISA Kit ET3102-1 Human TGF-beta1 ELISA Kit CU1001b Human uPA Chromogenic Activity Assay kit (Indirect Assay) CU1001a Human uPA Chromogenic Activity Assay kit (Direct Assay)上一篇:biotech trading partners下一篇:Biotek Instruments Inc.
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